Detecting 'decoy cells' by phase-contrast microscopy
نویسندگان
چکیده
منابع مشابه
Halo-free Phase Contrast Microscopy
We present a new approach for retrieving halo-free phase contrast microscopy (hfPC) images by upgrading the conventional PC microscope with an external interferometric module, which generates sufficient data for reversing the halo artifact. Acquiring four independent intensity images, our approach first measures haloed phase maps of the sample. We solve for the halo-free sample transmission fun...
متن کاملSegmentation of Individual Cells in Phase Contrast Microscopy Images
Acknowledgment The authors acknowledge the support of the GAUK, grant No. 914813/2013, GA JU 134/2013/Z, project CENAKVA (CZ.1.05/2.1.00/01.0024) and grant GAČR No. 13-29225S. The authors would also like to thank the staff of Working place of tissue culture certified laboratory at Nové Hrady, namely Monika Homolková and Šárka Beranová for their assistance with the manual segmentation of the cel...
متن کاملCell-sensitive phase contrast microscopy imaging by multiple exposures
We propose a novel way of imaging live cells in a Petri dish by the phase contrast microscope. By taking multiple exposures of phase contrast microscopy images on the same cell dish, we estimate a cell-sensitive camera response function which responds to cells' irradiance signals but generates a constant on non-cell background signal. The result of this new microscopy imaging is visually superi...
متن کاملHigh-resolution quantitative phase-contrast microscopy by digital holography.
Techniques of digital holography are improved in order to obtain high-resolution, high-fidelity images of quantitative phase-contrast microscopy. In particular, the angular spectrum method of calculating holographic optical field is seen to have significant advantages including tight control of spurious noise components. Holographic phase images are obtained with 0.5 mum diffraction-limited lat...
متن کاملStudies on Antibody - Producing Cells by Hemolytic Plaque Method and Phase Contrast Microscopy
By KIYoHuio, IRIMAJIIII, ATSUSHI Homocm, Yuji OKA IoTo AND ICHITA AMAKI W ITH THE RECENT DEVELOPMENT of immunologic chemistry studies of immunoglobulins have made a great progress. Yet the morphologic picture of antibody-producing cells has not been definitely elucidated because of various technical difficulties. Nonspecific reactions and difficulty in cell identification complicate the Coons’ ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nephrology Dialysis Transplantation
سال: 2004
ISSN: 0931-0509,1460-2385
DOI: 10.1093/ndt/gfh074